Ameyer@life.bio.sur:/sb.edu Department of Ecology and Evolution, and Program iu Genetics, State University of New York In the pressure cooker of UK university education I doubt ff will ever enjoy the luxury of being able to teach a module
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PolyDetector HRP/DAB Detection System (Bio SB, U.S.A.). Brieﬂy, tissues were rinsed in PBS and epitope retrieval was realized in a pressure cooker (121 C and 8 psi of pressure) using the ImmunoRe-triever Citrate Solution (Bio SB, U.S.A.) during
Antigen retrieval, using a pressure cooker (T-FAL Clipso). Samples were incubated for 10 min with immunodetector peroxidase block solution (Bio-SB Inc. Santa Barbara, CA.) (Bio-SB Inc. Santa Bar-bara, CA.), following manufacturer’s instructions, samples
BIO SB, Santa Barbara, CA, USA 123 J Neurooncol (2007) 84:159–166 USA) in a pressure cooker for 5 min, and rinsed again in PBS. Afterwards, endoge-nous peroxidase was blocked with 0.25% H 2O 2/distilled water for 15 min, and blocking with 3% BSA in PBS. The
In a pressure cooker. After being washed in phosphate-buffered saline (pH 7.4), slides were anti-ColIV (CIV22; Bio SB, Santa Barbara, USA: 1/20), anti-α-SMA (1st 4; Dako A/S, Denmark: 1/100) followed by the LSAB method (LSAB + kit System-HRP, Dako). All slides were subsequently exposed
Decloaking Chamber ™ NxGen biocare.net Digital Programmable Pressure System for Heat-Induced Epitope Retrieval (HIER) CE Listed, ETL approved U.S. patent number 6,580,056; June 17, 2003
In a pressure cooker (Decloaking Chamber) at 125 °C for 30 seconds. SOX10 [BC34] was diluted and optimized at 1:100, The importance of having your SOX on: role of SOX10 in the development of neural crest-derived melanocytes and glia.
pressure cooker, autoclave Bio SB “anti-hTERT” Monoclonal antibody 64 Summary † Immunostaining is a powerful, informative technique † Localization of a molecular moiety by use of an antibody † A variety of detection strategies
Immunostaining 101 July 19, 2013 † pressure cooker/autoclave *HIER = Heat Induced Epitope Retrieval HTTR = High Temp Target Retrieval 28 AR = Antigen Retrieval HIER Pros: Bio SB “anti-hTERT” Monoclonal antibody 69 Pitfalls
Carried out in a pressure cooker in 10 mM citrate buffer (pH 6.0) for 2 to 5min. (Bio SB 69 Santa Felicia Dr., Santa Barbara, CA 93117, USA) ready to use for 30 min, After further incubation with the secondary antibody (45
Min in a pressure cooker. Endogenous peroxi-dase inactivation was carried out with H 2O (Bio SB Inc. CA, USA) on the slides was used. Finally, Kim JW, Park NH, Kang SB, Song YS. Expression of MMP-2, MMP-9, and Urokinase-type Plasminogen Activator in Cervical Intra-
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